Table 5.
Primers used for construction of expression cassette acoA-nk-T7
| Primer | Sequence 5′→3′ | PCR product |
|---|---|---|
| NkF |
GC GAATTC GC GTGAGAAGCAAAAAATTG |
nk |
| NkR |
GC CTCGAG TTGTGCAGCTGCTTGTAC |
|
|
acoAF |
GC GAATTC TCAGTCAAACGATGCAG |
Fragment A |
|
acoAR |
AGC GCT CGC AGC CGC CGG TCC TGC |
|
|
acoA-NkF |
GAC CGG CGG CTG CGA GTG CTg tga gaa gca aaa aat tgt g |
Fragment B |
| T7R | GC GGATCC CAA AAA ACC CCT CAA GA |