Table 2.
Median values and interquartile ranges for pulmonary and myocardial mRNA expression in arbitrary units (A.U.)
| |
LVt, LPS (−) |
HVt, LPS (−) |
LVt, LPS (+) |
HVt, LPS (+) |
|---|---|---|---|---|
| (N = 6) | (N = 6) | (N = 5) | (N = 6) | |
| Pulmonary mRNA expression (A.U.) | ||||
|
HSP70 |
17*10-3 |
2.5*10-3 |
3.8*10-3 |
16*10-3# |
| (8.7-26*10-3) |
(0.050-8.2*10-3) |
(3.4-6.2*10-3) |
(8.4-27*10-3) |
|
| Myocardial mRNA expression (A.U.) | ||||
|
TLR2 |
0.10*10-3 |
0.10*10-3 |
0.10*10-3 |
0.25*10-3 |
| (0–0.22*10-3) |
(0–0.25*10-3) |
(0.10-0.25*10-3) |
(0.18-0.55*10-3) |
|
|
TNFα |
0.10*10-3 |
0.15*10-3 |
0.20*10-3 |
0.45*10-3 |
| (0–0.38*10-3) |
(0.075-1.6*10-3) |
(0.05-0.35*10-3) |
(0.10-0.63*10-3) |
|
|
IL6 |
0 |
0.05*10-3 |
0.10*10-3 |
0.35*10-3 |
| (0–0.35*10-3) |
(0–3.1*10-3) |
(0–7.0*10-3) |
(0–4.7*10-3) |
|
|
IL1β |
0.45*10-3 |
1.3*10-3 |
1.6*10-3 |
1.9*10-3 |
| (0.075-3.9*10-3) |
(0.38-15*10-3) |
(0.65-2.6*10-3) |
(0.83-4.9*10-3) |
|
|
CXCL1 |
17*10-3 |
12*10-3 |
21*10-3 |
119*10-3 ## |
| (2.73-57.8*10-3) | (3.8-67*10-3) | (16-37*10-3) | (71-225*10-3) | |
Relative expression levels of target genes were calculated relative to the housekeeping gene β-actin with the formula 2(Ct (β-actin)- Ct (target gene)). LPS lipopolysaccharide, LVt low tidal volume (Vt) ventilation, HVt high Vt ventilation, HSP70 heat shock protein 70, TLR2 toll like receptor 2, TNF α tumor necrosis factor α, IL6 Interleukin 6, IL1β Interleukin 1β, CXCL1 chemokine (C-X-C motif) ligand 1. The interaction between LPS and HVt ventilation increased pulmonary HSP70 (#, P<0.001) and myocardial CXCL1 mRNA expression (##, P=0.013).