Figure 3.

Immunofluorescent staining of spermatogonial cells in clusters. Integrin-α6 (A–C), integrin-β1 (G–I), Oct4 (M–O), and Thy-1 (S–U) were detected within the cluster cells (first column). DAPI staining in the second column and a merge of FITC and DAPI in the third column. Negative controls were treated without primary antibody (D–F, J–L, P–R and V–X).

Note: Scale bars = 200 μm.

Abbreviations: DAPI, 4,6-diamidino-2-phenylindole; FITC, fluorescein isothiocyanate; Itgα6, integrin-α6; Itgβ 1, integrin-β 1; Oct4, octamer-binding transcription factor 4; Thy-1, thymus cell antigen 1.