Table 2.
Detection of BTV RNA by RT-qPCR in blood and tissues of lambs.
|
Lamb |
Virus |
Blood sample |
Blood sample |
Blood sample |
Spleen |
Mesenteric lymph nodes |
Brain |
Cotyledons |
|---|---|---|---|---|---|---|---|---|
| 11 | 22 | 33 | ||||||
| 1 |
None |
- |
- |
- |
- |
- |
- |
- |
| 2 |
|
- |
- |
- |
- |
- |
- |
- |
| 3 |
|
- |
- |
- |
- |
- |
- |
- |
| 4 |
|
19.32 |
21.3 |
18.5 |
24.86 |
29.71 |
27.28 |
N.D. |
| 5 |
BTV-2 wt |
- |
- |
- |
- |
- |
- |
28.93 |
| 6 |
|
18.08 |
N.D. |
N.D. |
21.87 |
26.83 |
24.98 |
23.54 |
| 7 |
|
- |
- |
- |
- |
- |
- |
N.D. |
| 8 |
|
20.82 |
18.88 |
18.91 |
23.48 |
30.84 |
26.87 |
24.25 |
| 9 |
|
- |
- |
- |
- |
- |
- |
29.94 |
| 10 |
|
- |
- |
- |
N.D. |
N.D. |
N.D. |
25.96 |
| 11 |
BTV-8 wt |
- |
- |
- |
- |
- |
- |
35.05 |
| 12 |
|
- |
- |
- |
- |
- |
- |
N.D. |
| 13 |
|
- |
- |
- |
- |
- |
- |
28.49 |
| 14 |
|
- |
- |
- |
- |
- |
- |
27.65 |
| 15 |
|
N.D.4 |
N.D.4 |
N.D.4 |
19.99 |
27.41 |
25.96 |
22.72 |
| 16 |
|
- |
- |
N.D. |
- |
- |
- |
N.D. |
| 17 |
BTV-8 rg |
- |
- |
- |
- |
- |
- |
- |
| 18 |
|
- |
- |
- |
- |
- |
- |
33.13 |
| 19 |
|
- |
- |
- |
- |
- |
- |
25.72 |
| 20 |
|
- |
- |
- |
- |
- |
- |
34.26 |
| 21 |
|
- |
- |
- |
- |
- |
- |
N.D. |
| 22 |
|
- |
- |
- |
- |
- |
- |
26.61 |
| 23 |
BTV-2 rg |
19.53 |
18.12 |
19.67 |
26.23 |
31.8 |
29.47 |
22.16 |
| 24 |
|
- |
- |
- |
- |
- |
- |
31.02 |
| 25 |
|
- |
N.D. |
N.D. |
N.D. |
- |
- |
N.D. |
| 26 |
|
22.47 |
21.12 |
20.24 |
26.57 |
30.62 |
30.17 |
25.91 |
| 27 |
|
21.1 |
19.28 |
19.29 |
26.08 |
30.75 |
29.47 |
24.49 |
| 28 | - | - | - | - | - | - | N.D. |
1: Blood sample 1 was taken soon after birth before colostrum was received.
2: Blood sample 2 was normally taken at 3dpi but was delayed in some samples.
3: Blood sample 3 was normally taken at 7 dpi but was delayed in some samples.
4: Lamb 15 died at birth.
- indicates no Ct value obtained by 50 cycles.
N.D. = not determined.