Skip to main content
. Author manuscript; available in PMC: 2013 Dec 3.
Published in final edited form as: Neuron Glia Biol. 2011 Mar 4;6(4):10.1017/S1740925X11000019. doi: 10.1017/S1740925X11000019

Fig. 2. Cultured Gja1Jrt/+ astrocytes and cardiac myocytes express Cx43-positive GJs and are dye coupled.

Fig. 2

Astrocytes (A,B) and cardiac myocytes (E,F) were cultured from individual Gja1Jrt/+ mice or their Gja1+/+ littermates and either injected with LY (arrowheads indicate the injected cell) or fixed, immunostained for Cx43, and imaged by epifluorescence (C,D) or confocal (G,H) microscopy. Note that Gja1Jrt/+ and Gja1+/+ astrocytes and cardiac myocytes were extensively labeled with LY, and have Cx43-positive (red) GJ plaques on apposed cell membranes (arrows). Scale bars: 10 μm.