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. Author manuscript; available in PMC: 2013 Dec 3.
Published in final edited form as: Neuron Glia Biol. 2011 Mar 4;6(4):10.1017/S1740925X11000019. doi: 10.1017/S1740925X11000019

Fig. 6. Reduced Cx43 in the CNS of Gja1Jrt/+ mice.

Fig. 6

These are immunoblots of individual cerebra, cerebella and spinal cords of P40 Gja1Jrt/+ mice and their Gja1+/+ littermates. Ten μg of protein/lane was separated by electrophoresis, transferred to a membrane, probed with a rabbit antiserum against Cx43, then re-probed with mouse monoclonal antibody against GADPH, to show that the loading was comparable. The level of Cx43 is lower in Gja1Jrt/+ cerebella (B) and spinal cords (C), but not cerebra (A). The larger bands of Cx43 in the cerebra are likely phosphorylated isoforms (Manias et al., 2008). The Gja1Jrt/+ and Gja1+/+ samples were on the same membrane, but the intervening lanes were spliced out.