Table 1. Dual whole-cell patch clamp recordings.
Neuro2A cells were transiently transfected to express the indicated constructs and were paired as shown. GJ coupling was measured in nS. As expected, Cx43 homotypic pairs (WT/WT) were well coupled, as were pairs of cells in which both cells expressed Cx43WT, regardless of whether one (WT + G60S/WT) or both (WT + G60S/WT + G60S) cells also expressed G60S. Cell pairs expressing G60S alone (G60S/G60S) showed no coupling (significantly different from WT/WT at P < 0.05). Only one heterotypic Cx43G60S/Cx43WT pairing showed any coupling; the other six cell pairs showed no coupling.
| WT/WT | G60S/G60S | WT/G60S | WT + G60S/WT + G60S | WT + G60S/WT | |
|---|---|---|---|---|---|
| n | 10 | 8 | 11 | 6 | 13 |
| Mean (nS) SEM | 40.8 | 0.0 | 2.56* | 39.5 | 37.2 |
| 7.74 | 0.0 | 2.52 | 5.88 | 5.19 | |
| P (versus WT/WT) | – | <0.05 | <0.05 | NS | NS |
*
All due to one true outlier.