Table 1.
List of primers used to amplify and sequence gene fragments in this study
| Primer name | Primer sequence (5’-3’) | Amplified region |
|---|---|---|
| PGLU |
GAARAAYCANTRTTGTATTCAAC |
Cytb |
| PGLU-TAT** |
GAARAAYCANTRTTGTATTCAACTAT |
Cytb |
| MVZ-15 [27] |
GAACTAATGGCCCACACWWTACGNAA |
Cytb |
| HEM-CB1-5’ |
CCATCCAACATCTCAGCATGATGAAA |
Cytb |
| CYTBTN5Fv2 |
CATATTTAGGRGAAACACTTGTTCA |
Cytb |
| CYTBTYPHmR |
GTCKGGGYTAGAATTAATTCCTG |
Cytb |
| EurTXCRThr |
GYCAATGTTTTTCTAAACTACAACAGCATC |
Cytb |
| METf L4437 |
AAGCTTTTGGGCCCATACC |
ND2 |
| COIr H5934 |
TGCCAATATCTTTGTGATTTGTT |
ND2 |
| ND2f L5002* |
AATCAACCACAAATCCGAAAAAT |
ND2 |
| ASNr H5692* |
TTAGGTATTTAGCTGTTAA |
ND2 |
| MVZ 202** [28] | GCGTCWGGGTARTCTGAATATCGTCG | ND2 |
ND2 includes adjacent Ala and Trp tRNA genes.
*indicates primer was only used for sequencing, and not PCR.
** indicates external primer for nested PCR.