Figure 1.
Analysis of the expression of the entire M. truncatula GS gene family in several organs of the plant. The organs analysed include roots, de-nodulated roots from N2-grown plants (Roots-DN), 14 day old nodules, leaves, stems, cotyledons from light (Light Cot) and from dark grown seedlings (Dark Cot), flowers, pods and green seeds. A. Quantification of MtGS1a, MtGS1b, MtGS2a and MtGS2b transcripts by qRT-PCR. GS expression was normalized to that of the housekeeping gene MtElf1-α. B. Western blot analysis of GS polypeptides using anti-Glnγ antibody [27] recognizing both the cytosolic (GS1) and plastid-located (GS2) polypeptides and a specific anti-GS2 antibody. Equal amount of protein were loaded on each lane, 30 μg for the anti-Glnγ antibody and 10 μg for the anti-GS2 antibody. C. Quantification of GS activity on soluble protein extracts. The results presented in this figure are representative of at least four biological replicates.