Figure 2.

Optimization of TAG production from xylose by R. opacus Xsp8 in batch-culture fermentations. (a) Central composite experimental design matrix defining xylose and (NH₄)₂SO₄ concentrations. X₁, xylose concentration (g L^-1); X₂, (NH₄)₂SO₄ concentration (g L^-1). The strain was inoculated in the modified defined medium supplemented with gentamicin in Sixfors-bioreactors. The data for TAG production as fatty acids represent the maximum values during 10 days of cultivation. (b) Response surface plot of the effect of xylose and (NH₄)₂SO₄ concentrations on fatty acid production. Curves and points represent predicted values and experimental data, respectively.