Table 1.
Read statistics of 454 cDNA sequences after quality filtering
| Reads | Number of reads | Bases |
|---|---|---|
| BR |
1,182,916 |
425,618,400 |
| BS |
1,177,524 |
426,564,503 |
| Aligned* |
2,200,571 |
785,888,510 |
| (93.2%) |
(92.2%) |
|
| Assembled* |
1,981,006 |
54,877,004 |
| Partially aligned* |
219,291 |
- |
| Singletons* |
133,795 |
- |
| Repeats* |
471 |
- |
| Outliers* | 24,280 | - |
*Definitions of these Newbler de novo assembly terms can be found in Additional file 5: Text S1.
Sequence libraries of one resistant bulk (BR) and one susceptible bulk (BS) each containing pooled cDNA of 16 individual F2 plants were generated to perform the 454 sequencing.