Figure 5.

The ParS/ParR system negatively controls phenazine production and motility. A. Pyocyanin production of wild-type (PAO1), and mutant strains in AB medium + 2% CAA 24 h post-inoculation. B. Pyocyanin production by different strains in vitro. Bacterial strains were grown in AB medium +2% CAA for 24 h at 37°C with shaking. Pyocyanin was extracted and quantified at OD₅₂₀. Data points represent the means of three replicates ± standard deviations. Similar results were obtained in at least two independent experiments. CP, CR and CS indicate the wild type, parR and parS mutants harboring the complementation plasmid pKT2-parSR, respectively. C. Comparison of motility among wild-type, parS and parR mutants. Cells were inoculated on the surface of a motility plate containing 0.4% agar as described previously. Plates were incubated at 28°C for 36 h, during which the displacement (diameter) of the outermost edge of the movement was measured. The experiment was performed three times in triplicate. Errors bars were smaller than the symbols.