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. Author manuscript; available in PMC: 2013 Dec 3.
Published in final edited form as: J Neurosci Methods. 2005 Sep 19;151(2):10.1016/j.jneumeth.2005.07.010. doi: 10.1016/j.jneumeth.2005.07.010

Fig. 1. Image processing.

Fig. 1

(A) The user selects a rectangular region (dotted line) encompassing the entire neuron in the last image of the series. Debris and undesired image features can be excluded from subsequent analysis with a polygonal regioning tool (solid line). (B) The polygonal cropped image is then contrast-adjusted, thresholded, and filtered to generate the binary image used by the search algorithm.