Figure 3.

Modulation of protein expression of signal transduction molecules in case-derived cell cultures treated with pharmacological inhibitors: LY294002 (A), PD98059 (B), U0126 (C) and SB203580 (D).

Notes: The data represent the mean and SD of three independent experiments, each performed in triplicate. Expression of all tested signaling molecules was consistently inhibited after exposure to PI3K inhibitor LY294002 (P<0.05). MEK-1 inhibitor PD98059 determined also statistically significant inhibition on Case B, at moderate levels, while on Case A it was effective only for fewer molecules. JNK MAP-kinase and IκBα expressions were affected similarly by U0126 and PD98059. *P<0.05; **P<0.01.

Abbreviations: PI3K, phosphatidylinositol-3′-kinase; SD, standard deviation; vs, versus; CREB, cAMP response element-binding protein; ERK, extracellular signal-regulated kinase; JNK, jun amino-terminal kinase.