Figure 5.

Real-time impedance analysis of cell proliferation under PI3K inhibition.

Notes: Five thousand U87 cells (A), Case A derived culture cells (B), Case B derived culture cells (C), and Case B relapse derived culture cells (D) were plated on collagen-coated cells and left to adhere for 2 hours. After the plating period, vehicle comprising 1/400 DMSO or 25μM LY294002, PI3K inhibitor, were added to the wells; cells were further incubated in standard cell culture conditions for 48 hours. LY294002 inhibited proliferation in all experimental setups, yet at different amplitudes and times. Proliferation CI were normalized after an initial 2-hour plating period. Readings were collected every minute for the first 2 hours, then every 15 minutes for the remainder of the experiment. Statistical analysis was carried out using Student’s t-test (two-tailed) for normalized cell indexes at 24 hours *(P<0.01). The data represent the mean and SD of three independent experiments.

Abbreviations: CI, cell indexes; DMSO, dimethyl sulfoxide; SD, standard deviation.