Figure 6.

Real-time impedance analysis of cell proliferation under MEK-1 inhibition.

Notes: Five thousand U87 cells (A), and Case B relapse derived culture cells (B) were plated on collagen-coated cells and left to adhere for 2 hours. After the plating period, vehicles containing 1/400 DMSO or MEK-1 inhibitor to a final concentration of 25 μM were added to wells and cells were further incubated in standard cell culture conditions for 48 hours. Both cell types displayed inhibited proliferation upon treatment. Proliferation CI were normalized after an initial 2-hour plating period. Readings were collected at every minute for the first 2 hours, then every 15 minutes for the remainder of the experiment. Statistical analysis was carried out using Student’s t-test (two-tailed) for normalized cell indexes at 24 hours (P<0.01)*. The data represent the mean and SD of three independent experiments.

Abbreviations: CI, cell indexes; DMSO, dimethyl sulfoxide; SD, standard deviation.