Table 1.

Differentially expressed proteins in Vitamin C treated AGS cells identified by MALDI-TOF/MS

Spot no.	Swissprot entry name1	Protein name1	Accession number1	Theoretical/ experimental Mr (kDa)2	Theoretical/ experimental pI value2	Protein score3	Sequence coverage (%)/peptides matched	Fold change
Up - regulated proteins
2	TNIP2_HUMAN	TNFAIP3-interacting protein 2	Q8NFZ5	49.24/91	6.03/5.83	56	15/7	2.3
3	XIRP1_HUMAN	Xin actin-binding repeat-containing protein 1	Q702N8	199.64/96	5.78/6.48	59	11/13	2.2
6	TXND5_HUMAN	Thioredoxin domain-containing protein 5	Q8NBS9	48.28/49	5.63/5.38	81	22/10	2.9
7	CE290_HUMAN	Centrosomal protein of 290 kDa	O15078	290.89/28	5.75/6.04	69	9/16	8.5
8	PRDX4_HUMAN	Peroxiredoxin-4	Q13162	30.75/24	5.86/5.43	68	38/6	3.0
9	CE290_HUMAN	Centrosomal protein of 290 kDa	O15078	290.89/23	5.75/5.21	60	8/15	6.4
Down - regulated proteins
1	DPP3_HUMAN	Dipeptidyl peptidase 3	Q9NY33	82.88/100	5.02/4.86	110	26/12	4.0
4	DCTN2_HUMAN	Dynactin subunit 2	Q13561	44.32/51	5.10/4.94	122	39/13	2.3
5	IF4A1_HUMAN	Eukaryotic initiation factor 4A-I	P60842	46.35/48	5.32/5.31	175	47/17	2.1
10	PSB6_HUMAN	Proteasome subunit beta type-6	PSMB6	25.57/21	4.80/4.64	104	39/10	4.4
11	EIF3K_HUMAN	Eukaryotic translation initiation factor 3 subunit K	Q9UBQ5	25.33/21	4.81/4.46	101	37/8	3.6
12	PSA5_HUMAN	Proteasome subunit alpha type-5	P28066	26.57/24	4.74/4.31	91	41/8	2.0
13	IF6_HUMAN	Eukaryotic translation initiation factor 6	P56537	27.10/23	4.56/4.13	86	50/7	3.8
14	1433Z_HUMAN	14-3-3 protein zeta/delta	P63104	27.9/25	4.73/4.36	93	40/10	2.6
15	1433S_HUMAN	14-3-3 protein sigma	P31947	27.87/25	4.68/4.26	150	58/12	2.2
16	1433E_HUMAN	14-3-3 protein epsilon	P62258	29.33/26	4.63/4.27	131	93/12	2.3
17	EF1B_HUMAN	Elongation factor 1-beta	P24534	24.92/26	4.50/4.12	130	50/11	3.3
18	TPM4_HUMAN	Tropomyosin alpha-4 chain	P67936	28.62/27	4.67/4.28	94	25/10	3.1
19	TPM3_HUMAN	Tropomyosin alpha-3 chain	P06753	32.86/27	4.68/4.37	95	20/11	2.7
20	PCNA_HUMAN	Proliferating cell nuclear antigen	P12004	29.09/29	4.57/4.28	127	40/10	2.5

¹Entry name, protein name and accession number from SWISS-PROT database identified by MALDI-TOF/MS.

²Theoretical molecular weight (kDa) and pI from SWISS-PROT database; the experimental values of pI and MW for every isoelectric spot were calculated using Progenesis samespots software (Nonlinear Dynamics Ltd., Newcastle, UK).

³Score is −10*log (p), where p is the probability that the observed match is a random event, Protein scores greater than 56 are significant (p<0.05).