Table 1.
Reference loci and target genes included in the experiments
| Reference | Name | NCBI RefSeq | Function | Amplicon size (bp) | Exon location |
|---|---|---|---|---|---|
| ACTB* |
Beta-Actin |
NM_001101 |
Cytoskeletal protein |
173 |
7p22 |
| B2M§ |
Beta-2-microglobulin |
NM_004048 |
Major histocompatibility complex I component |
86 |
15q21-q22 |
| GAPDH* |
Glyceraldehyde-3-phosphate dehydrogenase |
NM_002046 |
Oxidoreductase in glycolysis and gluconeogenesis |
111 |
12p13 |
| HMBS* |
Hydroxymethyl-bilane synthase |
NM_000190 |
Porphyrin metabolism and heme synthesis |
64 |
11q23 |
| HPRT1* |
Hypoxanthine phosphoribosyl-transferase 1 |
NM_000194 |
Purine synthesis in salvage pathway |
94 |
Xq26 |
| PPIA* |
Peptidylprolyl isomerase A (Cyclophilin A) |
NM_021130 |
Catalyzation of cis-trans isomerization of proline imidic peptide bonds |
71 |
7p13 |
| RPL13A* |
Ribosomal protein L13A |
NM_012423 |
60S ribosomal subunit structural component |
126 |
19q13 |
| SDHA* |
Succinate dehydrogenase complex, subunit A |
NM_004168 |
Electron transporter in citric acid cycle and respiratory chain |
86 |
5p15 |
| TBP* |
TATA box binding protein |
NM_003194 |
General transcription factor for RNA polymerase II |
89 |
6q27 |
| UBC* |
Ubiquitin C |
NM_021009 |
Involved in protein degradation |
133 |
12q24 |
| YWHAZ§ |
Tyrosine 3-monooxygenase / tryptophan 5-monooxygenase activation protein, zeta polypeptide |
NM_003406 |
Signal transduction; binds to phosphorylated serine residues on several signaling molecules |
94 |
2p25 |
| AluSq |
Alu repeats, subfamily Sq |
||||
| POU5F1§ |
POU class 5 homeobox 1, Oct4 |
NM_002701.4 |
Marker for embryonic stem cell pluripotency |
130 |
6p21 |
| NANOG* | Nanog homeobox | NM_024865.2 | Marker for embryonic stem cell pluripotency | 109 | 12p13 |
*exon-spanning primer pair; §forward and reverse primer localized in the same exon.