Figure 4. Proliferation of Giardia trophozoites in 90% DMEM/10%Giardia media at 3 starting densities.

A) Giardia trophozoites were added to 24-well plates at three different starting densities: 50,000, 100,000, or 300,000 parasites/cm² in the absence and presence of Caco-2 cells. B) Growth curve of Giardia co-cultured with Caco-2 cells over 48 hours at the three starting densities. Values are expressed as the mean ± SEM. (n = 3).