Table 3.

Down-regulated gene functions in cell cultures at 12^th passage as compared to cells at 4^th passage

Class	Gene	Description	Functions	Ref
TP53 NODE	TP53	Major tumor protein suppressor	Cell cycle; Apoptosis; Senescence	[41,42]
	UBE2D3	Member of E2 ubiquitin conjugating enzyme family	Protein degradation machinery	[43]
	MAP2K3	Protein kinase activated by environmental stress	Gene expression regulation; Mitosis; Cellular differentiation; Cellular proliferation; Apoptosis; Senescence	[44]
	ZHX1	Zing finger and homeoboxes gene family	Maintenance of TP53 gene silencing	[45]
	XPO1	Exportin 1	Protein trafficking; Localization of cyclin b; Localization of MPAK; Nuclear export of TP53	[46]
	PIN1	Phosphorylation-dependent prolyne isomerase	Cellular differentiation; Cellular proliferation; Immune response; Mitosis	[47,48]
	CDC27	Component of anaphase promoting complex	Mitosis; Ubiquitination	[49]
	ANAPC2	Anaphase promoting complex	Cell cycle control; Ubiquitination	[50]
Cell cycle regulation and differentiation	TYMS	Thymidylate syntase	DNA replication and repair; Mitosis	[51]
	FGFR3	Fibroblast growth factor receptor 3	Mitosis; MSCs differentiation	[52,53]
	NCAPD2	Subunit of condensin I	Mitosis; Proliferation	[54,55]
	MITF	Transcription factor	Cell cycle regulation; Gene expression; Differentiation	[56-58]
Oxidative stress response	RPS3	Ribosomal protein (40s subunit)	DNA damage repair; Kinase activity on NFkB complex	[59]
	NFE2L2	Human basic leucine zipper transcription factor	Oxidative stress response	[60]
	HSP1	Heat-shock protein 1	Protein folding	[61-63]
NFkB signalling	IKBKG	Regulatory subunit of IKK complex	NFkB activation	[64]
	MYL5	Myosin light chain	NFkB activation	[65,66]
Apoptosis	SLC6A4	Membrane serotonin transporter	Apoptosis	[67]
Histonic modification	HAT1	Histone acetyltransferase B	Histone acetylation; Aging; Cellular differentiation	[68]
	HIST1H3C	Member of Histone H3 family	Transcriptional regulation; Cellular differentiation	[69]