Figure 2.

Effect of papain on the expression of VEGF-receptor 2. HUVEC were seeded into fibronectin coated 8-well chamber slides and cultured in EGM. After 24 hours, medium was changed and 10 ng/mL VEGF or a combination of 10 ng/mL VEGF and 10 μg/mL papain. After 1 hour, cells were fixed with 2% paraformaldehyde and stained with primary mouse antibody against VEGFR2 and FITC labeled goat anti mouse secondary antibody (Panel B). Cell nuclei were counter stained using DAPI. Unspecific mouse IgG served as isotype control (Panel A). Although there is slight unspecific binding of secondary antibody visible on the isotype control, VEGFR2 was clearly present on cells after treatment with 10 μg/mL papain.