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. 2013 Sep 25;13:126. doi: 10.1186/1471-2377-13-126

Figure 2.

Figure 2

Determination of the optimal Gdn-SCN concentration for denaturation of samples. (A) Two 10% sCJD brain homogenates (■ and ●) were treated with increasing concentration of Gdn-SCN (0-3 M). Samples were 10 times diluted and the fragment PrP226* was measured by V5B2/EM20-b sandwich ELISA. (B) The effect of Gdn-SCN on the capturing antibody V5B2 was determined using rec PrP226* which was first mixed with 3 M Gdn-SCN 1:1 (v/v), than diluted 5x and further serially diluted prior to loading on a plate (■). Controls were titrated non-denatured recombinant PrP226* (●) and PBS (▲).