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. 2013 Sep 25;13:126. doi: 10.1186/1471-2377-13-126

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Copyright © 2013 Dvorakova et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Analyzes of brain homogenate samples with PrP226* assay. 23 CJD and one GSS brain homogenate (A, C, E) and 10 non-CJD brain homogenates (B, D, F) were analyzed with V5B2/EM20-b DELFIA under native conditions (A, B) or after denaturation with 3 M Gdn-SCN for 15 min at 60°C (C, D). Absolute values are represented as counts per minute (cpm), measured by time-resolved fluorescence. The D/N ratio is represented in (E) and (F). Error bars were calculated from two independent experiments (E). Absolute measurements of native and denatured samples are presented without error bars, as an example only, as they differed significantly between repeated experiments, due to the sensitivity of DELFIA to slight variations in the protocol.