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. 2013 Sep 17;14:629. doi: 10.1186/1471-2164-14-629

Figure 4.

Figure 4

Target gene validation and expression analysis. (A) Mapping of target mRNA cleavage sites using 5’-RLM-RACE. The arrows indicate the cleavage sites and the number shows the frequency of the clones sequenced. (B) Quantitative real-time PCR analysis of the target mRNAs. The data represent the mean values ± SD of three replicates. The dots represent the corresponding abundance on the right axis in four small RNA libraries. TC241907: ARF8 (auxin response factor 8); TC236756: MYB; TC259543: CaMBP (calmodulin-binding protein); TC229767: ACCD (acetyl-CoA carboxylase beta subunit); TC251689: CIBP (calcium ion-binding protein); TC246633: LRR-RLK (leucine-rich repeat receptor-like protein kinase); TC277934: SPL9 (squamosa promoter-binding protein-like 9); TC279526: GHHB8 (HD-Zip protein 8).