Figure 3. RT-qPCR results for RNA isolated with a high-performance biofluids-specific protocol (Exiqon). Cq and standard deviation of RT-qPCR results for five plant miRNAs and one exogenous spike-in RNA are shown for plasma from subjects 67X (left) and HL26 (right) obtained at the pre-prandial (0) time point and at 1, 4 and 12 h post-prandial. Asterisk indicates a time point for which no reactions amplified for that miRNA. In the bottom panels, highly consistent recovery is shown for a miRNA (cel-miR-39) that was spiked in to the RNA isolation reactions to assess variability in the isolation technique that could have affected results.