Figure 1. Output screenshots of mirTools 2.0. The output includes: (1) basic statistics, such as length distribution, percentage of reads aligned to the reference genome, chromosome distribution, functional categories of reads and ncRNA distribution; (2) known miRNA, putative novel miRNA, miRNA isoforms and modification; (3) miRNA-targeted genes and functional annotation based on GO, the KEGG pathway and the PPI network; (4) expression information of other types of ncRNA, such as rRNA, tRNA, snRNA, snoRNA and piRNA; and (5) differentially expressed ncRNAs between two cases, two experimental groups or among multiple samples.