Figure 2. Mecp2 is bound to the 5′-end genomic loci of the lncRNAs AK081227 and AK087060 in wild-type mouse brain and lost in Mecp2-null mice. Semiquantitative chromatin immunoprecitation assay for a fraction of the total DNA (5%) (input), an antibody anti-H3 total (IP control), an antibody anti-Mecp2 and a negative control (mouse IgG) on the 5′-end genomic loci of the lncRNAs Xist (A), AK081227 (B) and AK087060 (C). The regions amplified by PCR were represented as straight lines.