Table 1.
MIC data for lacticin 3147, polymyxin B and polymyxin E alone and in combination
|
Organism |
MIC (μg/ml) |
||||||
|---|---|---|---|---|---|---|---|
| |
Lacticin 3147 |
Polymyxin B |
Polymyxin E |
Lacticin 3147/ |
FIC |
Lacticin 3147/ |
FIC |
| Polymyxin B | Polymyxin E | ||||||
|
Salmonella Typhimurium UK1 |
924 |
0.0586 |
0.0586 |
924/0.015 |
1.25d |
924/0.0073 |
1.125d |
|
Salmonella Typhimurium LT2 |
231 |
0.3125 |
0.4688 |
No MIC |
>4e |
No MIC |
>4e |
|
Cronobacter sakazakii DPC 6440 |
>924 |
0.3125 |
0.3125 |
57.75/0.0781 |
0.250 (<0.312)*a |
57.75/0.0195 |
0.062 (<0.125)*a |
|
E. coli 0157:H- |
231 |
0.0586 |
0.0781 |
28.875/0.0073 |
0.250a |
28.875/0.0049 |
0.188a |
|
E. coli DH5α |
462 |
0.0781 |
0.0781 |
28.875/.0098 |
0.188a |
28.875/0.0098 |
0.188a |
|
E. coli EC101 |
462 |
0.0781 |
0.0781 |
14.4375/.0391 |
0.5a |
28.875/0.0098 |
0.188a |
|
E. faecium DO |
0.9625 |
>375 |
>375 |
0.9625/23.4375 |
1c |
0.9652/23.4375 |
1c |
|
B. cereus 8079 |
3.85 |
187.5 |
375 |
1.925/23.4375 |
0.62b |
3.85/375 |
2d |
| S .aureus 5247 | 15.4 | 187.5 | >375 | 7.7/46.875 | 0.75b | 15.4/23.4375 | 1c |
FIC figures have been calculated as a result of triplicate experiments and indicate asynergy, bfor partial synergy, cadditive effects, dindifference, and eantagonism between the combined antimicrobials. *FIC index which includes the reduction in lacticin 3147 MIC from the highest level tested to that which achieves an MIC in the presence of polymyxin.