Figure 2. Dynamic Network Analysis (DyNA) of inflammatory mediators produced by normoxic and hypoxic mouse hepatocytes.

Primary hepatocytes from wild-type mice were cultured under normoxic or hypoxic conditions (1–72 h) followed by measurement of inflammatory mediators in both lysates and supernatants and lysates as described in the Materials and Methods . After data normalization, DyNA during each of the following five time frames: 1–3 h, 3–6 h, 6–24 h, 24–48 h, and 48–72 h was performed for both lysates and supernatants as indicated. Panels show a summary of the DyNAs representing the most connected inflammatory mediator “nodes” for both normoxia (A) and hypoxia (B) lysates and supernatants. Each mediator's node size is proportional to the number of connections it has in a given time interval. (C) Stacked bars representing the total number of connections for each inflammatory mediator over all time intervals.