. Author manuscript; available in PMC: 2014 Aug 6.

Published in final edited form as: Biochemistry. 2013 Jul 23;52(31):10.1021/bi400572a. doi: 10.1021/bi400572a

Table 1.

Regioselectivity of SgcF wild-type and variants towards (R)- and (S)-4 reported as percent enantiomeric excess (% ee) of the product (R)-5, % retaining activity, and the difference in free energy of activation between hydrolysis at each epoxide carbon (ΔΔG^‡).^a

	(R)-4 substrate			(S)-4 substrate
EH	% ee of (R)-5	% retain	ΔΔG^‡ (kJ/mol)^b	% ee of (R)-5	% retain	ΔΔG^‡ (kJ/mol)^b
SgcF wild-type	40.1 ± 2.5	70	−2.10	96.6 ± 0.1	1.7	−10.03
SgcF (W236Y)	65.6 ± 3.6	83	−3.90	90.8 ± 3.4	4.6	−7.62
SgcF (Q237M)	79.6 ± 5.2	90	−5.44	95.9 ± 0.6	2.1	−9.58
SgcF (W236Y/Q237M)	88.5 ± 0.5	94	−6.93	28.4 ± 4.9	36	−1.45

Errors represent standard deviations of three measurements.

Calculated according to the equation: ΔΔG^‡ = -RTln[major diol]/[minor diol]^29,30