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The LF is a strong inhibitor for TrCel7A on both 14C-BC and MUL substrates. (A) 14C-BC (0.25 mg ml-1) was incubated with the mixture of 0.25 μM TrCel7A, 0.025 μM EG (TrCel5A), and 0.1 μM N188BG at 25°C in the presence of no LF (□), N188BG-treated LF (♦), or sulfuric acid–treated LF (▲). (B) MUL (5 μM) was incubated with 10 nM TrCel7A at 35°C in the presence of the original LF (◊) or the N188BG-treated LF (♦).
