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. 2013 Sep 21;6:135. doi: 10.1186/1754-6834-6-135

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Copyright © 2013 Kont et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Separation of inhibitory compounds from the LF. The LF was fractionated using size exclusion chromatography. Fractions were analyzed for the reducing groups (white columns) and the inhibitory power against TrCel7A on MUL (gray columns) using 100 times diluted fractions. HPLC chromatograms of the selected fractions are shown in the insets. Fractions with elution volumes between 155.0 and 172.5 ml (Mw range of 1.0 – 3.0 kDa, indicated with red arrows) were pooled and purified using HPLC.