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Observation of chloroplasts within subepidermal palisade cells. (A-H) Chloroplasts in subepidermal palisade cells observed using the method established here. Jigsaw-puzzle-shaped epidermal cells are seen in (A). Focal depth is gradually deepened from (A) to (H). Leaf samples are from 21-day-old plants. Arrowheads indicate focused chloroplasts. (I) Magnified view of the boxed region in (C). Focused chloroplasts are outlined in black. Bars = 50 μm (A-H) and 10 μm (I).
