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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Clin Immunol. 2013 Aug 23;149(3):10.1016/j.clim.2013.08.008. doi: 10.1016/j.clim.2013.08.008

Figure 6.

Figure 6

Histone peptide cocktails suppress expression of type I IFN genes and IFN-induced genes in lupus PBMC. (A) PBMC from active SLE patients were treated with or without peptide cocktail-2 (C2) for 13 days before mRNA of type I IFN induced genes were quantified by qPCR. The bars represent the mean ± SEM from five individual experiments. (B) The upper panel shows results with whole PBMC from inactive SLE patients who were taking very low doses of steroids. The PBMC were cultured for five days in the presence or absence of peptide cocktail-2 and then stimulated with TLR9 ligand CPG-containing oligonucleotide (ODN) 2216 for 4 hours before type I IFN genes mRNA level were quantified by qPCR. The lower panel shows the qPCR results of same experiments as done in upper panel but T cells were depleted from the PBMC before cell culture. The results show mean ± SEM, from four individual experiments. * p < 0.01, * * p < 0.001.