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. 2013 Sep 26;1(4):350–359. doi: 10.1016/j.stemcr.2013.08.003

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© 2013 The Authors

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-No Derivative Works License, which permits non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Normalized Chromosome Copy Numbers

The absolute copy number for the autosomes is calculated by multiplying the NRQ values by 2 according to this formula: Copy number = calibrator copy number X NRQ or (2^−ddCq). A copy number of 2 reflects normal autosome ploidy, and a copy number of 3 reflects trisomy. The absolute copy number of chromosome Y equals the NRQ value. A copy number of 1 reflects normal ploidy, a copy number of 0 reflects loss of chrY, and a copy number of 2 reflects duplication of chrY. A dotted line marks the border between mESCs with previously determined (known) and unknown karyotypes at the time of qPCR analysis. See also Figure S1.