Table 1.
Cytogenetic Karyotypes of Mouse ESC Lines Used for qPCR
| Cell Line | ISCN | Cell Line | ISCN |
|---|---|---|---|
| Normal | Trisomy 8 | ||
| ESC 3 | 40,XY [15] | ESC 1 | 41,XY,+8 [15] |
| ESC 5 | 40,XY [14] | ESC 2 | 41,XY,+8 [15] |
| ESC 19a | 40,XY [15] | ESC 4 | 41,XY,+8 [15] |
| ESC 24 | 40,XY [14] | ESC 7 | 41,XY,+8 [15] |
| ESC 25 | 40,XY [14] | ESC 9 | 41,XY,+8 [15] |
| ESC 31a | 40,XY [12] | ESC 20 | 41,XY,+8 [14] |
| ESC 32 | 40,XY [13] | ESC 27 | 41,XY,+8 [13] |
| ESC 33 | 40,XY [15] | ESC 28 | 41,XY,+8 [14] |
| ESC 52a | 40,XY [14] | ESC 29 | 41,XY,+8 [15] |
| ESC 53a | 40,XY [15] | ESC 30 | 41,XY,+8 [15] |
| ESC 54a | 40,XY [15] | ESC 51 | 41,XY,+8 [13] |
| 31% Trisomy 1 | Trisomy 8 and Extra chrY | ||
| ESC8b | See Table S1 | ESC12 | 42,XYY,+8 [15] |
| Loss Of chrY | Trisomy 8 and 11 | ||
| ESC 10 | 39,X,-Y [15] | ESC 13 | 42,XY,+8,+11 [14] |
| ESC 14 | 39,X,-Y [15] | ESC 16 | 42,XY,+8,+11 [13] |
| ESC 15 | 39,X,-Y [14] | ESC 18 | 42,XY,+8,+11 [15] |
| ESC 17 | 38∼39,X,-Y [16] | ||
| ESC 21 | 39,X,-Y [15] | ||
| ESC 22 | 39,X,-Y [15] | ||
| ESC 23 | 39,X,-Y [14] | ||
The following lines had unknown cytogenetic karyotypes and were used to validate our qPCR approach: ESC 6, ESC 11, and ESC 34–ESC 50. Karyotypes of lines ESC 40, ESC 45, and ESC 48 were confirmed by DAPI-banded karyotyping after obtaining the qPCR results. Karyotypes were the following: ESC 40, 37∼40,XY[20]; ESC 45, 38∼39,X,-Y[15]/40,XY[1]; ESC48, 39∼40,XY[15]. See also Table S1.
a
These cell lines produced germline animals.
b
ESC8 is of mixed genetic background and was derived in the lab.