. 2013 Sep 26;1(4):350–359. doi: 10.1016/j.stemcr.2013.08.003

Table 2.

In Silico and Empirical Evaluation of the qPCR Primer Sets^a

Chr	Location	Gene	Primers (5′-3′)	Amplicon Length (bp)	RTprimerDB ID	Standard Curve
Chr	Location	Gene	Primers (5′-3′)	Amplicon Length (bp)	RTprimerDB ID	Slope	E (%)	r²	Linear Dynamic Range (ng)	LOD (ng)
1	172956826–172956929	olfr16	GAGTTCGTCTTCCTGGGATTC	103	8651	−2.904	120	0.997	0.84–13.5	0.09^b
1	172956826–172956929	olfr16	TAATGATGTTGCCAGCCAGA	103	8651	−2.904	120	0.997	0.84–13.5	0.09^b
7	98145342–98145482	omp	GCCCACTTGATTCCCTGA	140	8652	−2.907	120	0.997	0.84–13.5	0.07
7	98145342–98145482	omp	GCATCTGCTGGGTCAGGTCC	140	8652	−2.907	120	0.997	0.84–13.5	0.07
8	83541895–83542057	olfr370	CACTATGGATGTGCCTCTTTTATCT	162	8653	−3.137	108	0.999	0.84–13.5	0.08
8	83541895–83542057	olfr370	TACCCTATGAAGAGCTGATTTGAAG	162	8653	−3.137	108	0.999	0.84–13.5	0.08
11	73395777–73395941	olfr1	TCTATGCCCTGTTCCTGGTC	164	8654	−3.439	95	0.996	0.84–13.5	0.02
11	73395777–73395941	olfr1	CAACTTGGGCATTGTGACAG	164	8654	−3.439	95	0.996	0.84–13.5	0.02
X	78085543–78085693	obp1a	GGATCAGAATTATGGATCATGTG	150	8655	−2.994	115	0.994	0.84–13.5	0.15
X	78085543–78085693	obp1a	GATCATGAGAAGGGGAAGGA	150	8655	−2.994	115	0.994	0.84–13.5	0.15
Y	2663266–2663432	sry	CTCATCGGAGGGCTAAAGTG	166	8656	−3.235	103	0.985	0.84–13.50	0.28^c
Y	2663266–2663432	sry	AAGCTTTGCTGGTTTTTGGA	166	8656	−3.235	103	0.985	0.84–13.50	0.28^c