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. 2013 Dec 3;8(12):e81928. doi: 10.1371/journal.pone.0081928

Figure 2. Effect of PARP inhibitor on poly-ADP-ribosylation (PAR) and protein nitration in transplanted kidneys.

Figure 2

(A) Immunoblot analysis of poly-ADP-ribosylated HistonH1. HistonH1 was immunoprecipitated with anti-histonH1 antibody, dissolved in sample buffer, subjected to electrophoresis and blotting. Poly-ADP-ribose was detected by anti-PAR antibody. (B) Quantitative analysis of immunoblot samples (C) Representative images of nitrotyrosine immunohistochemistry. Brown color indicates nitrotyrosine positivity; scale bar: 50 µm. (D) Quantitative analysis of immunoblot samples. *: p<0,0001 untreated transplanted kidneys compared to others. ANOVA, Bonferroni post hoc test. Mean±SD. All details described under Materials and Methods.