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. 2013 Dec 3;8(12):e81586. doi: 10.1371/journal.pone.0081586

Figure 1. In vitro antitumorigenic activity of peonidin-3-glucoside or cyaniding-3-glucoside.

Figure 1

(A). Structures of hit compounds. (B) Effect of peonidin-3-glucoside and cyanidin-3-glucoside on HER2 and downstream signaling. BT474, MDA-MB-453, and HCC1569 cells were treated with or without peonidin-3-glucoside or cyanidin-3-glucoside for 6 h and whole-cell extracts were analyzed by western blotting with the indicated antibodies. (C) Peonidin-3-glucoside and cyanidin-3-glucoside treatments induce apoptosis in MDA-MB-453, BT474, and HCC1569 cells. Cells were treated with or without peonidin-3-glucoside or cyanidin-3-glucoside for 48 h and annexin V positive cells were counted using flow cytometry. (D) Peonidin-3-glucoside and cyanidin-3-glucoside treatments increase caspase 3/7 activity in MDA-MB-453, BT474, and HCC1569 cells. Cells were treated with or without peonidin-3-glucoside or cyanidin-3-glucoside for 48 h and cell viability and caspase 3/7 activity was measured as per the manufacturer's instructions. Data were normalized as caspase 3/7 activity divided by cell viability. Data represent mean ± SEM. *p<0.05, **p<0.01.