Figure 2. CpG island methylation in the αA-crystallin promoter was similar in age-related cataracts and high-myopic cataracts classified as Lens Opacity Classification System III Grade Nuclear Color 2–3.

(A) Representative Pyrosequencing results of the Control, Age-Related Cataract (ARC) Nuclear Color (NC) 2–3 and High-Myopic Cataract (HMC) NC2–3 Groups. (B) Anterior segment photos of patients in each group. (C) The six selected CpG islands in the ARC NC2–3 (44±8.3%) and HMC NC2–3 (43.7±3.8%) Groups displayed higher methylation compared with the Control Group (34±6.1%). (D) Percentage of methylation at each individual CpG site in each group. (*Statistically significant differences were found between the Control and ARC NC2–3 Groups; ^&statistically significant differences were found between the Control and HMC NC2–3 Groups; *^,&all p<0.05. However, no statistically significant differences were found between the two cataract groups.)