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. Author manuscript; available in PMC: 2014 Nov 28.
Published in final edited form as: J Control Release. 2013 Aug 23;172(1):10.1016/j.jconrel.2013.08.016. doi: 10.1016/j.jconrel.2013.08.016

Fig. 5.

Fig. 5

LMWP-mediated cellular uptake by tumor cells of: (A) recombinant gelonin (rGel), (B) gelonin-LMWP chemical conjugate (cG-L), or (C) recombinant gelonin-LMWP chimera (rG-L). CT26 cells were treated with rhodamine-labeled gelonin samples for 3 hr at 37°C in 5% CO2 humidified incubator. After stringent wash with 10 mg/mL heparin/PBS solution, nuclei were counter-stained with Hoechst 33342. Images of the cells were captured by different channels (brightfield (gray), Hoechst 33342 (blue) and rhodamine (gold)) by Nikon epifluorescence microscope. Merged images were obtained by overlapping images taken with Hoechst 33342 and rhodamine channels.