Table 3.
Reading Frame–Dependent Restoration of Luciferase Activity by the 8-nt Duplication
| Frame | Constructa | Luciferase Activityb(%) |
| 0 | PCMV- GAA …c | 0 |
| 0 | PCMV-ATG CTG CGG ACG CCG GGA CGC CGG GAC GCC GAA … |
100 |
| +1 | PCMV-ATG CTG CGG ACG CCG GGA CGC CGG GAC GCC AGA A… |
1.1 |
| +2 | PCMV-ATG CTG CGG ACG CCG GGA CGC CGG GAC GCC ACG AA… |
4.3 |
a
ATG codon following CMV promoter (PCMV) is second ATG of PEX7 (nt 31–33 of cDNA). Open reading frame of firefly luciferase starts at the second codon and is shown in bold. Underlined sequence denotes the 8-nt duplication
b
100% is 3.106 units luciferase activity normalized for β-galactosidase activity
c
Control construct without PEX7 sequence and without the luciferase ATG.