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. 2013 Dec 3;8(12):e81949. doi: 10.1371/journal.pone.0081949

Figure 6. Fatty acids treatment increase Kupffer cells ability on antigen presentation and activation of NKT cells.

Figure 6

Kupffer cells were isolated from wild-type C57BL/6 mice and treated with fatty acids (PA, 0.5mmol/L) and loaded with αGalCer (100ng/ml) overnight, and co-cultured with HMNC (served as NKT cells source) for 24h. Cells and culture supernatant were collected for detection of cytokine mRNA expression and protein levels. Increased expression of IFN-γand IL-4 indicated activation of NKT cells by αGalCer-loaded Kupffer cells. (A) IFN-γand IL-4 mRNA expression in co-culture system determined by real time PCR. (B) IFN-γand IL-4 levels in culture supernatant determined by ELISA. Values are means±SEM , *P<0.05, **P<0.01 versus Blank, # P<0.05 αGalCer group versus αGalCer+PA group, n=3 experiment.