Table 1.

Primers for Amplification, Sequencing, and RFLP Analyses^[Note]

Primer Pair	Locations in CRS	AnnealingTemperature(°C)	Polymorphisms at/in
L29/H408	8–29/429–408	54	HVS-II
L394/H902	375–394/922–902	60	+663HaeIII (663)
L2796/H3274	2777–2796/3293–3274	57	3010, 3206
L3179/H3674	3160–3179/3693–3674	59	+3391HaeIII (3394)
L4499/H5099	4480–4499/5118–5099	60	+4831HhaI (4833), 4715
L4887/H5442	4866–4887/5461–5442	56	−5176 AluI (5178A), 5231, 5417
L7356/H7805	7337–7356/7824–7805	57	−7598HhaI (7598, 7600)
L8215/H8297	8196–8215/8316–8297	57	9-bp deletion
L9794/H10164	9774–9794/10181–10164	60	+9824 HinfI (9824)
L10170/H10660	10147–10170/10679–10660	59	10171–10659
L11338/H11944	11319–11338/11963–11944	53	11719
L12334/H12878	12315–12334/12897–12878	57	12705, 12358, 12372
L14054/H14591	14035–14054/14610–14591	57	14178, 14308, 14318, 14470
L14575/H15086	14556–14575/15105–15086	57	14766
L15391/H16048	15372–15391/16067–16048	58	15487T, 15784
L15996/H16498	15975–15996/16517–16498	60	HVS-I

Note.— PCR conditions were 94°C for 2 min, for denaturation; 94°C for 40 s; annealing temperature shown for 1 min, for amplification; and 72 °C for 1 min, for 35–40 cycles; incubation at 72°C for 5 min.