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. 2013 Sep 24;11:93. doi: 10.1186/1477-7827-11-93

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Copyright © 2013 Ko et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effects of OSM (20 ng/mL) on mRNA and protein expression of E-cadherin in extravillous cytotrophoblast cells (HTR8/SVneo). Total RNA was extracted from the cells, with or without OSM stimulation for 48 h, and mRNA levels were measured by real-time quantitative RT-PCR analysis (A). E-cadherin mRNA levels were standardized to GAPDH mRNA levels. Results are presented as means ± SEM of 3 independent experiments. The representative effects of OSM on E-cadherin as observed from the western blots are shown in relation to the control (48 h) (B). Quantitative results (C) of western blots were normalized to GAPDH and presented as a fold change over the controls (untreated = 1). Data shown are the means ± SEM of 3 independent experiments compared to the control (*p < 0.05).