Figure 5.

Electrophoretic mobility shift analysis of the −112G/A polymorphic site. A, Oligonucleotide sequences containing G (−112G) or A (−112A) at −112 bp (boldface), which were used as a probe or a competitor, are shown. The consensus sequence for C/EBP obtained by Transcription Factor Search (Heinemeyer et al. 1998) is underlined. B, A specific DNA-protein complex, formed between a nuclear protein in NCI-H441 cells and a ³²P-labeled −112G fragment, was subjected to competition analysis in the presence of increasing concentrations (one to ninefold) of unlabeled −112G or −112A oligonucleotide, as a competitor. A representative result is shown. C, Graphic demonstration of the results obtained in B. -----⧫----- = −112A oligonucleotide; ——□—— = −112G oligonucleotide. Band intensity was quantitated using a PhosphoImager and ImageQuant program (Molecular Dynamics). Each value represents the mean of three independent experiments ± SD.