Table 5. Singleplex KASPar SNP validation assay of a set of 100 discovered SNP markers.
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Parental lines specific SNP*
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|---|---|---|---|---|
| Diversity Set | DH12075 x PSA12 | V8 x Express | Both populations | |
| Individuals tested | 25 | 30 | 29 | - |
| Total SNP tested** | 100 | 19 | 37 | 44 |
| Amplification type | ||||
| No amplification | 12 | 0 | 4 | 8 |
| Monomorphic | 9 | 1 | 5 | 6 |
| Multiple Loci | 8 | 2 | 0 | 6 |
| Polymorphic*** | 71 | 16 | 28 | 24 |
| % PA | 88 | 100 | 89 | 82 |
| % PS | 71 | 84 | 78 | 55 |
Abbreviations: PA = Positive Amplification; PS = Polymorphic SNPs.
* Represents markers specifically designed from SNPs that showed polymorphism for a specific set of reference mapping parental lines. For instance, polymorphisms between the spring type parents, (DH12075 and PSA12); polymorphisms between the winter type parents, (V8 and Express); and polymorphisms detected for both sets of parental lines at the same SNP locus.
** Out of the 100 SNPs tested 73 SNPs were A genome-specific and 27 were C genome-specific.
*** Polymorphic SNPs also include two dominant (presence/absence of the tested allele) marker types.