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. 2013 Nov 15;45(11):e58. doi: 10.1038/emm.2013.121

Figure 2.

Figure 2

Human salivary gland stem cells (hSGSCs) have mesenchymal stem cell (MSC)-like differentiation potential. (a) Adipogenic differentiation of hSGSCs determined by staining with Oil Red O. (b) Adipogenic gene expression was confirmed by reverse transcriptase-PCR (RT-PCR) after 3 weeks of differentiation. (c) Osteogenic differentiation of hSGSCs determined by staining with Alizarin Red. (d) Osteogenic gene expression was confirmed by RT-PCR after 3 weeks of differentiation. (e) Chondrogenic differentiation of hSGSCs determined by staining with Alcian Blue. (f) Chondrogenic gene expression was confirmed by RT-PCR after 3 weeks of differentiation. β-Actin was used as a loading control. Agc, aggrecan; BSP, bone sialoprotein; ColII, collagen type II; LPL, lipoprotein lipase; OC, osteocalcin; PPARγ, proliferator-activated receptor-γ Runx2, runt-related transcription factor 2. Bar, 200 μm.