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. 2013 Nov 15;45(11):e58. doi: 10.1038/emm.2013.121

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Copyright © 2013 KSBMB.

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/

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Human salivary gland stem cells (hSGSCs) were differentiated into amylase-expressing cells. (a) Experimental schedule for differentiation into amylase-expressing cells. (b) After 21 days of differentiation, amylase protein expression was detected by western blotting with an anti-amylase antibody. (c) After 21 days of differentiation, amylase RNA expression was detected by reverse transcriptase-PCR analysis. (d) Immunocytochemistry analysis was conducted to confirm amylase protein expression. Bar, 100 μm. DAPI, 4′-6-diamidino-2-phenylindole.