A, left, specific mRNA content (by competitive PCR before and after transcription inhibition for 40 h with 5 μg/ml actinomycin D) in human embryo kidney 293 cells transfected with either wild-type (white columns) or 1484insG (black columns) cDNA. A, right, data from the left panel are recalculated as % decrease after treatment with actinomycin D. Data are mean ± SEM of the results of three independent experiments. B, Representative competitive PCR for both wild-type (wt) and 1484insG transfected cells.